Isolation of DNA from saliva of betel quid chewers using Treated Cards
Pub2.pdf
Salvador, J.M. and De Ungria, M.C.A.
DNA Analysis Laboratory, Natural Sciences Research Institute, University of the Philippines, Quezon City

Journal of Forensic Science (in press). Betel quid (BQ) chewing, a common tradition in tropical areas, often poses a problem during collection and DNA analysis of buccal samples from many indigenous communities for population genetic studies and in forensic analysis of chewed BQ residues. This study evaluated the use of FTA™ card, a chemically treated filter paper, in collecting buccal samples from long-term BQ users and subsequent PCR-based analysis using nine STR markers. A low overall success rate of amplification was observed in the samples extracted
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large number of European as well as North and South American populations are available online (Y-STR Haplotype Reference Database YHRD ). The most recent multi-center effort has led to the establishment of an Asian YHRD which was made available in January 2002. All databases are maintained at the Institute of Legal Medicine, Humboldt-University, Berlin and will soon be fused with a global repository of Y haplotypes that include populations from all continents.